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目的探讨补肾与活血序贯干预对月经模型小鼠子宫内膜修复的影响及可能作用机制。方法将140只雌性C57BL/6J小鼠随机分为假手术组(40只)、模型组(50只)、序贯干预组(50只),模型组和序贯干预组采用切除小鼠双侧卵巢后行激素序贯处理复制小鼠月经模型。序贯干预组在激素序贯处理的同时序贯给予补肾方及活血方,每天相应药物2 ml/100 g灌胃,共计给药14天。模型组、假手术组灌胃等量蒸馏水。在孕酮撤退后24 h、32 h、40 h、48 h、72 h对各组小鼠诱导侧(右侧)子宫角进行形态学观察及评分,并检测各组小鼠子宫Cxcl 2蛋白的表达。结果与假手术组比较,模型组小鼠子宫内膜形态学评分在24 h、32 h、40 h、48 h显著降低(P<0.05);与模型组比较,序贯干预组小鼠子宫内膜形态学评分在24 h、32 h、40 h、48 h显著升高(P<0.05)。与假手术组比较,模型组Cxcl 2蛋白表达在各个时间点均升高(P<0.05);与模型组比较,序贯干预组Cxcl 2蛋白表达在各个时间点均升高(P<0.05)。结论补肾与活血序贯干预可促进月经模型小鼠子宫内膜修复,其作用机制可能与调控Cxcl 2蛋白表达有关。
Objective To investigate the effect of sequential intervention of tonifying kidney and promoting blood circulation on the endometrial repair of menstrual model mice and its possible mechanism. Methods Forty-four female C57BL / 6J mice were randomly divided into sham operation group (n = 40), model group (n = 50) and sequential intervention group (n = 50) Ovarian reproductive hormone sequential treatment of mouse model of menstruation. Sequential intervention group in the sequential treatment of hormone sequential administration Bushen Fang and Huoxue Fang, the corresponding drug 2 ml / 100 g orally, a total of 14 days. The model group and the sham-operated group were given the same amount of distilled water. Morphological observation and scoring were performed on the induced (right) uterine horn in each group at 24 h, 32 h, 40 h, 48 h and 72 h after progesterone withdrawal, and the levels of Cxcl 2 protein expression. Results Compared with the sham operation group, the endometrial morphological score of the model group decreased significantly at 24 h, 32 h, 40 h and 48 h (P <0.05). Compared with the model group, the intrauterine Membrane morphological score at 24 h, 32 h, 40 h, 48 h significantly increased (P <0.05). Compared with sham operation group, the expression of Cxcl2 protein in model group increased at all time points (P <0.05). Compared with model group, the expression of Cxcl2 protein in sequential intervention group increased at each time point (P <0.05) . Conclusion The sequential intervention of tonifying kidney and promoting blood circulation can promote the repair of endometrium in mice with menstruation. The mechanism may be related to the regulation of Cxcl2 protein expression.