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目的:探讨高脂诱导营养性肥胖大鼠中医证候属性,并分析该模型在红细胞膜上的生物学差异。方法:SD大鼠71只,随机分为普食组(10只)和高脂组(61只),分别喂饲普通饲料和高脂饲料,连续喂养9周。模型复制成功后,观察大鼠表观特征;取模型成功大鼠10只进行体质量、Lee’s指数、脂体比检测;取血测定甘油三酯(TG)、胆固醇(CHO)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)的水平;测定全血黏度、血浆黏度以及红细胞膜组分相关指标,并与普食组进行比较。结果:该种造模方法成模率为82%,且该模型具有中医痰湿证的表观特征;与普食组比较,高脂组大鼠体质量以及脂体比较具有极显著性差异(P均<0.01),Lee’s指数显著升高(P<0.05);甘油三酯(TG)含量显著升高(P<0.05),高密度脂蛋白(HDL-C)含量显著降低(P<0.05),全血黏度在200s-1切变率以及30s-1切变率下,高脂组显著升高(P<0.05),血浆黏度显著升高(P<0.05),红细胞膜上Na+-K+-ATP酶活力、超氧化物歧化酶(SOD)活力均极显著降低(P<0.01),丙二醛(MDA)含量显著升高(P<0.05)。结论:高脂诱导营养性肥胖大鼠模型的中医证候属性可为痰湿证,该模型可能由于Na+-K+-ATP酶及SOD活力的降低,MDA含量的升高导致全血黏度发生改变。
OBJECTIVE: To investigate the TCM syndromes of fatty obesity rats induced by high fat and to analyze the biological differences of the model on erythrocyte membrane. Methods: Seventy-one SD rats were randomly divided into general diet group (n = 10) and high fat diet group (n = 61), fed with normal diet and high fat diet respectively for 9 weeks. After successful replication of the model, the appearance characteristics of the rats were observed. Ten rats in the model were taken for body weight, Lee’s index and lipofection. Blood samples were taken for determination of triglyceride (TG), cholesterol (CHO), high density lipoprotein (HDL-C) and low density lipoprotein (LDL-C) were measured. The whole blood viscosity, plasma viscosity and erythrocyte membrane components were measured and compared with those of the general diet group. Results: The molding rate of the model was 82%, and the model had the obvious features of phlegm - dampness syndrome. Compared with the general diet group, there was a significant difference in body weight and body fat between the high fat diet group and the control group (P <0.01), and Lee’s index increased significantly (P <0.05). The triglyceride content increased significantly (P <0.05) and the content of HDL-C decreased significantly The whole blood viscosity at 200s-1 shear rate and 30s-1 shear rate significantly increased (P <0.05) and the plasma viscosity increased significantly (P <0.05) ATPase activity and superoxide dismutase (SOD) activity were significantly decreased (P <0.01), and MDA content was significantly increased (P <0.05). CONCLUSION: TCM syndromes of rats with nutritional fat induced by high fat diet may be phlegm-dampness syndrome. This model may be due to the decrease of Na + -K + -ATPase and SOD activity, and the increase of MDA content leads to the change of whole blood viscosity.