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目的:建立HPLC法同时测定养胃舒颗粒中绿原酸、牡荆素鼠李糖苷和橙皮苷3个成分的方法。方法:色谱条件:Agilent Zorbax SB-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%甲酸(B)体系为流动相进行梯度洗脱,流速为1.0m1·min~(-1),柱温为30℃,检测波长为330 nm。结果:3种成分均能达到基线分离,绿原酸、牡荆素鼠李糖苷和橙皮苷的进样量分别在0.020~0.403μg(r=0.999 7)、0.022~0.450μg(r=0.999 6)和0.009~0.189μg(r=1.000 0)内与峰面积呈良好的线性关系(n=6),平均加样回收率在95%~105%(n=6)。结论:该方法简便、快速、可靠、重复性好,可为养胃舒颗粒的质量控制提供参考依据。
Objective: To establish a HPLC method for the simultaneous determination of three components of rhamnoside and hesperidin in Yangweishu granules. METHODS: The chromatographic conditions were as follows: Agilent Zorbax SB-C 18 column (250 mm × 4.6 mm, 5 μm) with gradient elution with acetonitrile (A) -0.1% formic acid · Min -1, the column temperature was 30 ℃ and the detection wavelength was 330 nm. Results: The three components were able to achieve baseline separation. The injection volumes of chlorogenic acid and vitexin rhamnoside and hesperidin were 0.020 ~ 0.403μg (r = 0.999 7), 0.022 ~ 0.450μg (r = 0.999 (N = 6) and 0.009 ~ 0.189μg (r = 1.000 0). The average recoveries ranged from 95% to 105% (n = 6). Conclusion: The method is simple, rapid, reliable, reproducible and can provide reference for the quality control of Yangwei Shu granules.