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目的:分析循环滤泡辅助性T(c Tfh)细胞亚型与重症肌无力(MG)患者临床特点之间的关系。方法:横断面研究30例MG患者外周血c Tfh细胞各个亚型百分比和临床特点之间的关系。c Tfh细胞亚型的百分比是通过流式细胞术获取的。MG患者的临床特点包括性别、年龄、病程、胸腺情况、美国重症肌无力协会(MGFA)分型和重症肌无力量化(QMG)评分。结果:CD4~+CXCR5~+ICOS~+、CD4~+CXCR5~+PD-1~+和CD4~+CXCR5~+CXCR3-CCR6~+(Th17样)c Tfh细胞亚型百分比与QMG评分之间存在正相关关系;全身型重症肌无力(GMG)患者在CD4~+CXCR5~+ICOS~+、CD4~+CXCR5~+PD-1~+和Th17样c Tfh细胞亚型百分比要高于眼肌型重症肌无力(OMG)患者,OMG患者在CD4~+CXCR5~+CXCR3-CCR6-(Th2样)c Tfh亚型细胞百分比要高于GMG患者。c Tfh细胞各个亚型百分比与MG患者的性别、年龄、病程、胸腺情况均无显著相关性。结论:CD4~+CXCR5~+ICOS~+、CD4~+CXCR5~+PD-1~+和Th17样c Tfh细胞亚型百分比与MG病情严重性间存在正相关关系,提示c Tfh细胞与MG之间的密切关系。
Objective: To analyze the relationship between the clinical features of circulating follicular helper T (c Tfh) cell subtypes and myasthenia gravis (MG). Methods: The cross-sectional study of 30 cases of MG patients with peripheral blood c Tfh percentage of each subtype and clinical features. The percentage of cTfh cell subtypes is obtained by flow cytometry. Clinical features of patients with MG include gender, age, duration of disease, thymus status, American Association for Myasthenia Gravis (MGFA) classification and QMG score. Results: The percentages of CD4 ~ + CXCR5 ~ + ICOS ~ +, CD4 ~ + CXCR5 ~ + PD-1 ~ + and CD4 ~ + CXCR5 ~ + CXCR3-CCR6 ~ + (Th17-like) c Tfh cell subtypes and QMG score There was a positive correlation between the percentage of CD4 + CXCR5 + ICOS +, CD4 + CXCR5 + PD-1 + and Th17-like T cell subsets in patients with systemic myasthenia gravis (GMG) (OMG), the percentage of cells with CD4 ~ + CXCR5 ~ + CXCR3-CCR6- (Th2-like) c Tfh subtype in patients with OMG was higher than those with GMG. There was no significant correlation between the percentages of each subtypes of c-Tfh cells and the gender, age, course of disease and thymus of patients with MG. CONCLUSION: There is a positive correlation between the percentages of CD4 ~ + CXCR5 ~ + ICOS ~ +, CD4 ~ + CXCR5 ~ + PD-1 ~ + and Th17-like Tfh cell subtypes and the severity of MG, suggesting that the relationship between cTfh cells and MG Close relationship between.