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目的：建立神经生长因子活性鉴定方法，证实牛精液中含有丰富的神经生长因子。方法：采用牛精液粗提物体外无血清培养鸡胚背根神经节。结果：背根神经节长出繁茂的神经突生长晕，此无血清培养方法重复性好。结论：牛精液含有丰富的神经生长因子，背根神经节的无血清培养是神经生长因子活性鉴定的一种可靠方法。
OBJECTIVE: To establish a method for the identification of nerve growth factor activity and to confirm that bovine seminal fluid is rich in nerve growth factor. Methods: Chick embryo dorsal root ganglion was cultured in serum-free medium with bovine serum extract. Results: The dorsal root ganglion grew out of lush neurite outgrowth. This serum-free culture method had good reproducibility. CONCLUSION: Bovine seminal fluid is rich in NGF and serum-free culture of DRG is a reliable method for the identification of nerve growth factor activity.