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为观察发育期补充二十二碳六烯酸(DHA)对大鼠脑内脂肪酸代谢和部分神经递质的变化,将孕鼠分为4组:正常对照组(O)、添加DHA 的低(L)、中(M)、高(H)剂量组,于基础饲料中分别添加鱼油0、17.5、35和70m l/kg,实验组饲料中的DHA含量分别为7.5、15和30g/kg。以幼鼠脑匀浆中脂肪酸分析、大脑皮质和海马内单胺类神经递质5-羟色胺(5-HT)、去甲肾上腺素(NE)、多巴胺(DA)及肽类神经递质生长抑素(SS)作为检测脑神经生化代谢的指标,并监测孕鼠、仔鼠体重。结果表明:补充DHA的各剂量组幼鼠脑匀浆中DHA含量及海马中5-HT、DA、SS含量均显著高于对照组,但补充DHA各组仔鼠体重增长不同程度地受到抑制。结论:1.发育期补充DHA使大鼠脑内脂肪酸代谢发生变化,进而调节递质释放;2.对人和动物补充DHA的同时,应考虑EPA(C20v5n-3)、AA(C20v4 n-6 )等长链脂肪酸的适当含量和比例
In order to observe the changes of fatty acid metabolism and some neurotransmitters in rat brain during the period of DHA supplementation, the pregnant rats were divided into 4 groups: normal control group (O), DHA L, M and H respectively. Fish oil supplemented with 0, 17.5, 35 and 70 ml / kg of fish oil respectively, and the DHA content in the experimental group was 7.5, 15 and 30 g / kg, respectively. Fatty acid analysis of rat brain homogenates showed that the growth of neurotransmitters such as 5-HT, NE, DA and peptides in the cerebral cortex and hippocampus were increased Su (SS) as an indicator of brain neurobiochemical metabolism and monitoring of pregnant rats, offspring weight. The results showed that the contents of DHA and the content of 5-HT, DA and SS in hippocampus in each dose group of DHA were significantly higher than those in control group. However, the body weight gain in DHA-supplemented groups was inhibited to varying degrees. DHA supplementation during developmental changes fatty acid metabolism in the brain of rats, and then regulate the release of neurotransmitters.2. To supplement DHA with human and animals, we should consider the effects of EPA (C20v5n-3), AA (C20v4n-6 ) And other long-chain fatty acids appropriate content and proportion