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为了探索鸦片肽对生殖系统的确切影响,研究了β内啡肽的子宫结合特性。作者用Sprague-Dawley大鼠子宫制成子宫膜制剂,用~3Hβ内啡肽20mmol/L与此子宫膜制剂温育(30℃)15min及2h,以10％HAc中止反应,离心取得上清液,经Sephadex G50柱层析,发现~3Hβ内啡肽已分解为碎片,表明子宫膜制剂对β内啡肽有酶性降解作用,从而解释了以往文献报道未能显示子宫鸦片肽受体的原因。本文在使用丝氨酸蛋白酶抑制剂0.1mmol/L PMSF为保护剂的条件下,对子宫的β内啡肽受体进行受体分析,饱和实验表明,子宫膜悬液有β内啡肽结合效应,并对此种结合的特征作了描述和讨论。
To explore the exact effect of opiate peptides on the reproductive system, the uterine binding properties of beta endorphin were studied. The authors used the Sprague-Dawley rat uterus to make a uterine membrane preparation, which was stopped with 10% HAc by incubation with ~ 3Hβ endorphin at 20 mmol / L and this uterine membrane preparation (30 ° C) for 15 min and 2 h, and the supernatant was obtained by centrifugation , By Sephadex G50 column chromatography and found ~ 3Hβ endorphin has been broken down into pieces, indicating that the uterine membrane preparation of β-endorphin enzymatic degradation, thus explaining the previous literature failed to show the reasons for the uterine opiate peptide receptor . In this paper, the serine protease inhibitor 0.1mmol / L PMSF as a protective agent on the utero β endorphin receptor receptor analysis, saturation experiments showed that the uterine membrane suspension with β endorphin binding effect, and The characteristics of this combination are described and discussed.