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在 Ledos×中双 2号后代材料中发现 5株雄性不育株,经与中双 2号自交系 212B保持,育成了细胞质雄性不育系212A和保持系212B。通过人工套袋自交、剥蕾授粉自交及镜检观察,认为不育系212A属稳定型细胞质雄性不育系,产生的原因可能与低温时期的死蕾现象有关。恢保关系的研究表明,212A胞质雄性不育系与polCMS、陕2A CMS恢保关系相同。遗传研究表明,测交 F_2育性为可育与不育3:1分离;用恢复系回交(BC_1)后代全可育,无育性分离;用保持系回交,后代育性为可育与不育1:1分离。初步认为控制不育系的基因为S(rr),保持系基因为N(rr),恢复系基因为N(RR)或S(RR),属1对核基因控制的细胞质雄性不育系。
Five male sterile lines were found in the progenies of Ledos × Zhongshuang 2, and maintained in cytoplasmic male sterile line 212A and maintainer line 212B after being maintained with 212B inbred line Zhongzhong 2. Through artificially bagging self-pollination, self-pollination and pollination and microscopic observation, we concluded that CMS212A belongs to the stable cytoplasmic male sterile line, which may be related to the phenomenon of dead bud at low temperature. The research on the relationship between restorers and maintainers showed that 212A cytoplasmic male sterile lines had the same relationship with polCMS and Shan 2A CMS. Genetic studies showed that the fertility and fertility of tested F2 maize was segregated 3: 1 in fertility and sterile, and all the progeny of BC1 were segregated with fertility and infertility with backcrossing of restorer line (BC_1) And sterile 1: 1 separation. It is preliminarily considered that the genes controlling CMS are S (rr), the maintainer line is N (rr), and the restorer line is N (RR) or S (RR), which belongs to a cytoplasmic male sterile line controlled by nuclear gene.