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【目的】研究广西山银花当家品种红腺忍冬的组织培养,为红腺忍冬种苗工厂化生产提供技术支持。【方法】以红腺忍冬腋芽为外植体,用0.1%升汞溶液灭菌处理;以MS为基本培养基,探讨不同浓度6-BA、NAA和MET组合对芽诱导、增殖培养和根诱导的影响。【结果】较适宜外植体灭菌的处理为0.1%升汞溶液(加吐温-80)处理7min,外植体污染率为16.0%。春季取材的灭菌效果及腋芽萌发效果最好,冬季最差。较适宜的芽初代诱导培养基为MS+6-BA2.0mg/L,继代培养较适宜的培养基为MS+6-BA2.0mg/L+NAA0.1mg/L,生根培养较适宜的培养基为MS+NAA0.1mg/L+MET3.0mg/L。【结论】通过红腺忍冬腋芽培养,得到可用于生产的组培苗,该技术可应用于红腺忍冬种苗工厂化生产。
【Objective】 The purpose of this study was to investigate the tissue culture of Lonicerae Japonica L., a pioneer cultivar in Shannxihu, Guangxi, to provide technical support for the industrialized production of Lonicera rhizomania seedlings. 【Method】 The axillary buds of Lonicerae capitata were used as explants and sterilized with 0.1% mercuric chloride solution. MS medium was used to study the effects of different combinations of 6-BA, NAA and MET on bud induction, proliferation and root induction Impact. 【Result】 The explants were treated with 0.1% mercuric chloride solution (plus Tween-80) for 7 min. The explant contamination rate was 16.0%. Spring sterilization and axillary bud germination effect of the best, the worst winter. The most suitable initial induction medium for shoots was MS + 6-BA 2.0 mg / L, and the more suitable culture medium for subculture was MS + 6-BA 2.0 mg / L + NAA 0.1 mg / L. The base is MS + NAA 0.1 mg / L + MET 3.0 mg / L. 【Conclusion】 The tissue culture seedlings that can be used for production can be obtained through axillary bud culture of Lonicerae Japonica. This technique can be applied to the industrialized production of Lonicera rhizomania seedlings.