论文部分内容阅读
降低小麦中多酚氧化酶(PPO)活性,减缓面粉制品的褐化,是重要的育种目标之一。为了更好地服务于低PPO育种,本研究对检测PPO活性的原苯酚染色法进行了优化,更好地发挥了其鉴别力强、结果稳定、对种子活力伤害小等优点,便于育种者使用。苯酚染色和分子标记结果对比发现,染色结果可以很好地反映亲本(或高代)材料中PPO的基因型,特别在低PPO材料中吻合更好。对大量亲本和世代材料的籽粒染色发现,PPO不仅存在于种皮中,其活性还是由种皮基因型决定的,后代PPO性状表现出母性遗传和加性效应的特点,控制高PPO特性的两个主效基因之间具有明显的代偿作用。PPO性状遗传相对简单,纯合较快,F2以后籽粒的染色程度以单株为单位发生分离。尽管染色是针对种皮基因型的,但PPO基因的这些遗传特点和小麦的自交特性,使染色结果同样可以预测后代单株的分离前途。这一优化的籽粒染色法在低PPO育种中的有效性是可以肯定的。
Reducing the activity of polyphenol oxidase (PPO) in wheat and slowing the browning of flour products is one of the important breeding objectives. In order to better serve the low-PPO breeding, this study optimized the PPA method to detect the activity of PPO to better exert the advantages of strong discrimination, stable results and less damage to seed vigor, which is convenient for breeders to use . The comparison of phenol staining and molecular marker results showed that the staining results can well reflect the genotype of PPO in the parent (or high generation) materials, especially in the low PPO materials. The results showed that PPO not only existed in the seed coat, but also was determined by the seed coat genotype. The progeny PPO trait showed maternal heredity and additive effect. The control of high PPO properties A major gene between the obvious compensatory role. Genetic inheritance of PPO is relatively simple, homozygous fast, F2 after the degree of staining of the seeds to separate units. Although the staining is for the seed coat genotype, these genetic characteristics of the PPO gene and the selfing characteristics of the wheat make the staining results equally predictable for the future isolation of the offspring. The effectiveness of this optimized grain dyeing method in low PPO breeding is certain.