目的：探讨双黄连注射液２ 种指标成分含量的测定方法，控制其质量。方法：应用甲醇水溶液（ 磷酸调ｐＨ至２ ．７） 为流动相，在ＯＤＳＣ１８ 柱上梯度洗脱，外标法定量，绿原酸和黄芩苷２ 种指标成分含量可以同时定量。结果：３ 批样品中氯原酸的含量分别为０ ．７０ ％ ，０ ．７４ ％ 和０ ．７５ ％ （ｍｇｍｇ） ，黄芩苷的含量分别为１０ ．６７ ％ ，１０ ．２５ ％ 和１１ ．３８ ％ （ｍｇｍｇ） 。结论：该方法简便、快速、灵敏，适合于双黄连粉针剂的质量控制。 Objective: To investigate the determination of Shuanghuanglian injection two kinds of indicators of content, control its quality. Methods: Methanol  aqueous solution (adjusted to pH2.7 with phosphoric acid) was used as the mobile phase. The mobile phase was eluted with gradient elution on ODS C18 column. The contents of chlorogenic acid and baicalin were quantitatively determined by external standard method. Results: The contents of chlorogenic acid in three batches of samples were 0. 70%, 0. 74% and 0. 75% (mg? Mg), baicalin content of 10. 67%, 10. 25% and 11. 38% (mgmg). Conclusion: The method is simple, rapid and sensitive and suitable for the quality control of Shuanghuanglian injection.