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研究人粒细胞集落刺激因子(rhG-CSF)包涵体的复性方法。方法:采用我室发明的包涵体提纯新方法。结果:复性回收率可达90%以上,生物活性达天然比活的80%以上。结论:对rhG-CS F包涵体提纯和复性的研究,建立了rhG-CSF的复性新方法。
To study renaturation of human granulocyte colony-stimulating factor (rhG-CSF) inclusion bodies. Methods: A new method of inclusion body purification was invented in our laboratory. Results: The recovery rate of refolding can reach more than 90%, and the biological activity reaches more than 80% of the natural specific activity. Conclusion: The purification and refolding of rhG-CS F inclusion bodies were studied. A new renaturation method of rhG-CSF was established.