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目的：研究二甲基亚砜（ＤＭＳＯ）对人肺癌细胞凋亡的诱导作用。方法：用不同浓度的ＤＭＳＯ处理体外培养的人肺癌细胞Ａ５４９，应用普通光镜、荧光显微镜、ＭＴＴ分析方法和流式细胞技术（ＦＣＭ）检测肺癌细胞凋亡的形态学变化、细胞存活率、凋亡百分率和细胞周期分布的变化。结果：ＤＭＳＯ诱导Ａ５４９细胞核ＤＮＡ凝缩和核片段化，最后形成凋亡小体；随着ＤＭＳＯ浓度的增加和处理时间的延长，细胞存活率明显下降，其ＩＣ５０为３．２％；４％的ＤＭＳＯ处理细胞 １２ｈ，凋亡率高达３３．０％；同时Ｇ０／１期细胞明显憎加，Ｓ期和Ｇ２／Ｍ期细胞明显下降。结论：ＤＭＳＯ可诱导人肺癌细胞凋亡，并使细胞受阻于Ｇ０／１期而进入凋亡程序。
Objective: To study the induction of apoptosis of human lung cancer cells by dimethyl sulfoxide (DMSO). METHODS: Human lung cancer cells A549 were treated with different concentrations of DMSO in vitro. The morphological changes, cell viability, and apoptosis of lung cancer cells were detected by ordinary light microscope, fluorescence microscopy, MTT assay and flow cytometry (FCM). Percentage of death and changes in cell cycle distribution. RESULTS: DMSO induced nuclear DNA condensation and nuclear fragmentation of A549 cells, and finally formed apoptotic bodies; with the increase of DMSO concentration and treatment time, cell survival rate decreased significantly, and its IC50 was 3.2%; 4% After 12h treatment with DMSO, the apoptotic rate was as high as 33.0%. At the same time, cells in G0/1 phase increased significantly, and cells in S phase and G2/M phase decreased significantly. Conclusion: DMSO can induce apoptosis of human lung cancer cells, and the cells are arrested at G0/1 and enter apoptosis.