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核酸类小分子对植物具有增强根系活力,对麦芽根中核酸酶的提取及酶动力学性质进行研究,将酵母核糖核酸降解为核苷为代表的核酸类小分子,制成核酸叶面肥。首先对麦芽根中核酸酶的提取及酶学性质进行研究;其次选择适宜的RNA水解条件:RNA浓度2%,pH为5.6,温度为65℃,时间为3.5h;最后将水解液进行后处理:升温至100℃,煮沸10m in,灭酶,同时将pH调至7.0,放置3h,冷却沉降,除杂、浓缩、喷雾干燥得核酸叶面肥成品。通过研究取得核酸叶面肥的制备工艺。
Nucleic acids small molecules on the plant with increased root activity, the extraction of malt root nucleases and enzyme kinetic properties of the study, the degradation of yeast ribonucleic acid nucleotides as represented by small molecules, made of nucleic acid foliar fertilizer. Firstly, the extraction of malt root nuclease and its enzymatic properties were studied. Secondly, the suitable RNA hydrolysis conditions were as follows: RNA concentration 2%, pH 5.6, temperature 65 ℃, time 3.5h. Finally, the hydrolyzate was processed : Heated to 100 ℃, boiled 10m in, enzyme inactivation, while the pH adjusted to 7.0, place 3h, cooling and sedimentation, impurity removal, concentration, spray dried leaflet fertilizer finished. Obtained through the study of the preparation of nucleic acid foliar fertilizer.