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为了观察两种激动药的相互作用是否可出现相加、协同或假拮抗效应,作者对β-受体激动药多巴酚丁胺和肾上腺素进行了研究,实验分2期进行。第1期应用离体人淋巴细胞,测量肾上腺素和多巴酚丁胺刺激β-肾上腺素受体——腺苷酰环化酶环腺苷酸(cAMP)时cAMP的释放量;第2期观察多巴酚丁胺、肾上腺素及合用于主冠动脉搭桥术后病人的强心效应。 取10例健康人静脉血各50ml制备淋巴细胞提取液(70％T淋巴细胞和10％B淋巴细胞,细胞数为1×10~6个/ml)。淋巴细胞液中分别加入10~(-8)M～10~(-4)M多巴酚丁胺、10~(-9)M～10~(-5)M肾上腺素或两者并用,于27°
In order to observe whether there is additive, synergistic or pseudo-antagonistic effect on the interaction of two agonist drugs, the authors studied dobutamine and epinephrine on beta-agonist, and the experiment was divided into two phases. In phase 1, the release of cAMP was measured in vitro using adrenaline and dobutamine to stimulate cAMP, a β-adrenergic receptor-adenylyl cyclase. Phase 2 To observe dobutamine, epinephrine and co-administered coronary artery bypass graft in patients after cardiac effect. Twenty lymphocytes (70% T lymphocytes and 10% B lymphocytes, the number of cells was 1 × 10 ~ 6 cells / ml) were prepared from 50ml venous blood from 10 healthy volunteers. 10 ~ (-8) M ~ 10 ~ (-4) M dobutamine, 10 ~ (-9) M ~ 10 ~ (-5) M adrenaline, or both were used in combination with 27 °