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在强碱性条件下,KMnO4氧化酪氨酸产生自由基,自由基氧化Luminol产生强化学发光,酪氨酸浓度与相对发光强度呈线性关系。选用C18反相色谱柱、CH3OH、KH2PO4混合液为流动相,酪氨酸能很好分离。基于此,建立CL-HPLC检测酪氨酸的新方法,线性范围为1.2×10-3～5.0×10-2g/L、线性相关系数为0.9994、RSD为2.37%、检测限为3.3×10-4g/L。方法已于检测实际样品氨基酸口服液,并与UV光度法,结果一致。本文探讨了酪氨酸增强KMnO4-Luminol化学发光机理。
Under strong alkaline conditions, KMnO4 oxidizes tyrosine to generate free radicals, and free radical oxidation of Luminol produces strong chemiluminescence. The tyrosine concentration has a linear relationship with the relative luminescence intensity. Use C18 reversed-phase column, CH3OH, KH2PO4 mixture as mobile phase, tyrosine can be well separated. Based on this, a new method for the determination of tyrosine by CL-HPLC was established, with a linear range of 1.2 × 10-3 ~ 5.0 × 10-2g / L, a linear correlation coefficient of 0.9994, a RSD of 2.37% and a detection limit of 3.3 × 10- 4g / L. Method has been tested in the actual sample amino acid oral solution, and UV spectrophotometry, the results are consistent. This article explores the mechanism of tyrosine-enhanced KMnO4-Luminol chemiluminescence.