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目的探讨银杏叶提取物(GBE)对牙龈卟啉菌(Pg)膜泡(ECV)的影响。方法采用酶动力学方法,测定银杏叶提取物对膜泡生物活性的阻断作用和膜泡对人牙周膜成纤维细胞碱性磷酸酶活性的影响。结果与空白对照组相比,50μg/ml ECV可明显抑制人牙周膜成纤维细胞碱性磷酸酶(ALP)的活性(P<0.01),与膜泡组相比差异有统计学意义(P<0.01),且随着浓度升高而升高。结论膜泡生物活性可被银杏叶提取物有效阻滞,从而促使牙周膜成纤维细胞向成骨细胞转化,有利于病变的愈合。
Objective To investigate the effect of Ginkgo biloba extract (GBE) on Pg vesicles (ECV). Methods Enzyme kinetic method was used to determine the inhibitory effect of Ginkgo biloba extract on the biological activity of membrane vesicles and the effect of membrane vesicles on alkaline phosphatase activity of human periodontal ligament fibroblasts. Results Compared with the blank control group, 50μg / ml ECV could significantly inhibit alkaline phosphatase (ALP) activity of human periodontal ligament fibroblasts (P <0.01), and the difference was statistically significant (P <0.01), and increased with increasing concentration. Conclusion The bioactivity of membrane vesicles can be effectively blocked by Ginkgo biloba extract, which can promote the transformation of periodontal ligament fibroblasts into osteoblasts, which is good for the healing of the lesions.