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目的：探讨动脉成形术后血管平滑肌细胞（ＳＭＣ）表皮生长因子受体（ＥＧＦＲ）在再狭窄发生机理中的作用。方法：取正常家兔髂动脉、高脂血症兔髂动脉成形术后２周非狭窄段和狭窄段ＳＭＣ，分别用Ｎｏｒｔｈｅｒｎ杂交和免疫组化法检测３种静止期ＳＭＣ及增殖期再狭窄ＳＭＣ中ＥＧＦＲ的表达。结果：３种静止期ＳＭＣ均未见ＥＧＦＲｍＲＮＡ表达，而再狭窄ＳＭＣ受血清刺激后４ｈ即有ＥＧＦＲｍＲＮＡ表达，至指数生长期表达更明显。免疫组化显示ＥＧＦＲ蛋白质仅在指数生长期ＳＭＣ中显现阳性反应，其阳性颗粒定位于细胞膜。结论：ＥＧＦＲ表达增加与再狭窄过程中ＳＭＣ增殖有关。
Objective: To investigate the role of epidermal growth factor receptor (EGFR) of vascular smooth muscle cells (SMC) in the mechanism of restenosis after arterioplasty. Methods: Non-stenotic and stenotic segments of SMC were obtained from iliac artery and hyperlipidemic rabbits two weeks after normal iliac artery angioplasty. Northern hybridization and immunohistochemistry were used to detect the expression of three kinds of resting SMCs and proliferative restenosis SMCs EGFR expression. Results: No expression of EGFR mRNA was observed in the three quiescent SMCs. However, the expression of EGFR mRNA was significantly increased in the restenosis SMCs at 4 h after stimulation. Immunohistochemistry showed that EGFR protein showed positive reaction only in exponential growth phase in SMC, and positive particles located in the cell membrane. Conclusion: The increase of EGFR expression is related to the proliferation of SMC during restenosis.