【摘 要】
：
人β防御素2(Human beta defensin 2,HBD-2)是人体抗菌肽的重要分子,体外试验研究证明具有广谱抗微生物活性,为开展其在整体水平上的功能研究,建立HBD-2转基因小鼠模型.用分
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人β防御素2(Human beta defensin 2,HBD-2)是人体抗菌肽的重要分子,体外试验研究证明具有广谱抗微生物活性,为开展其在整体水平上的功能研究,建立HBD-2转基因小鼠模型.用分子克隆方法构建了带CMV启动子的HBD-2全长cDNA 真核重组表达质粒pCDNA3.1-HBD2,以此为摸板,PCR扩增出带CMV启动子和BGH polyA 尾的HBD-2基因片段,用显微注射技术将此微基因导入小鼠雄原核中,于M16培养液培养后经输卵管移植到受体鼠体内让其怀孕产生子代小鼠.PCR法检测外源基因在小鼠基因组中的整合,RT-PCR和免疫组化法检测HBD-2在小鼠组织的表达.PCR检测结果显示17只F0代转基因鼠中4只检测到阳性信号,RT-PCR和免疫组化检测显示HBD-2在其F1代转基因小鼠生殖道、呼吸道、泌尿道以及血管内皮等组织部位广泛表达.实验表明HBD-2基因已整合到小鼠基因组且广泛表达,为进一步研究HBD-2的生物学功能及基因调控提供了有用的整体动物模型.
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