论文部分内容阅读
对含硅羟基磷灰石(Si-HA)与人血清白蛋白(HSA)的吸附特性及动力学特征进行了研究,计算了表观活化能,用等温吸附曲线进行拟合。结果表明,该吸附属于Langmuir型。Si-HA和HA吸附HSA的表观活化能分别为21.28和35.57 kJ.mol-1,20℃下吸附反应的速率常数分别为1.48和1.34 min-1,饱和吸附量分别为25.34和21.34 mg.g-1,Si-HA具有比纯HA更好的吸附优势。通过XRD、FTIR及荧光光谱分析,探讨了HSA在Si-HA和HA表面的吸附作用机制,表明Si-HA与蛋白表面吸附反应是以化学吸附为主、包含物理吸附的混合吸附过程。从分子结构的角度揭示了Si-HA和HA吸附蛋白质性能的差异,为进一步探讨Si-HA和HA生物相容性提供了新途径。
The adsorption and kinetics characteristics of Si-HA and HSA were studied. The apparent activation energy was calculated and fitted by isothermal adsorption curve. The results show that the adsorption belongs to the Langmuir type. The apparent activation energies of adsorbed HSA on Si-HA and HA were 21.28 and 35.57 kJ · mol-1, respectively, and the adsorption constants of HSA and HA were 1.48 and 1.34 min-1 respectively at 20 ℃ and 25.34 and 21.34 mg, respectively. g-1, Si-HA has better adsorption advantages than pure HA. The adsorption mechanism of HSA on the Si-HA and HA surfaces was discussed by XRD, FTIR and fluorescence spectroscopy. The results showed that the adsorption reaction of Si-HA with the protein surface mainly consisted of chemisorption, including the process of mixed adsorption by physical adsorption. From the perspective of molecular structure, the difference of the properties of Si-HA and HA proteins was revealed, which provided a new way to further explore the biocompatibility of Si-HA and HA.