论文部分内容阅读
目的:建立HPLC法测定骨通贴膏中盐酸麻黄碱的含量及含量均匀度。方法:采用Kromasil C_(18)柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸溶液(5:95)为流动相,柱温为30℃,流速1.0 ml·min~(-1),检测波长为207 nm。结果:盐酸麻黄碱在0.052 7～2.635 0μg的范围内线性关系良好,r=1.000 0,平均回收率103.07%,RSD为1.70%(n=6)。结论:该方法操作简便,准确性、重复性好,可用于骨通贴膏中盐酸麻黄碱的含量及含量均匀度测定。
Objective: To establish an HPLC method for the determination of ephedrine hydrochloride in Gutong paste and its content uniformity. Methods: The mobile phase consisted of Kromasil C 18 column (250 mm × 4.6 mm, 5 μm) and acetonitrile-0.1% phosphoric acid solution (5:95) at a flow rate of 1.0 ml · min -1 , Detection wavelength of 207 nm. Results: Ephedrine hydrochloride showed good linearity in the range of 0.052 7 ~ 2.635 0 μg with r = 1.000 0, average recovery 103.07% and RSD 1.70% (n = 6). Conclusion: The method is simple, accurate, reproducible and can be used for the determination of content and evenness of content of ephedrine hydrochloride in Gutong paste.