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为了解外环境中致病性弧菌的污染状况,1990年6～9月,我们对采自外环境的148份样品,进行了致病性弧菌的检测。 采地表水40份;生活饮用水35份;列车餐车各类熟食品43份;处理后的医院污水30份。 地表水、生活饮用水、医院污水每份样品取200ml,加入10倍浓缩的碱性胨水20ml,37℃过夜培养,然后同时在改良麦康凯和SS平板上分离;并取1ml胨水培养液,接种于10ml氯化锶胨水中二次增菌培养过夜,在改良DC平板上分
To understand the external environment of pathogenic Vibrio pollution, from June to September 1990, we sampled 148 samples collected from the external environment for the detection of pathogenic Vibrio. Mining surface water 40; 35 drinking water; train dining car all kinds of cooked food 43; after treatment of 30 hospital sewage. Surface water, drinking water and hospital sewage were taken from each sample 200ml, 10 times the concentration of peptone 20ml concentrated, cultured overnight at 37 ℃, and then at the same time on the modified McCoy Kai and SS plate separation; and take 1ml peptone water broth , Inoculated in 10ml strontium peptone water secondary enrichment culture overnight in the modified DC plate