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研究了菥蓂提取物的体外抗氧化活性,筛选有效活性部位。通过对菥蓂水提物的有机溶剂萃取、D101大孔树脂分离纯化,制备得到8个部位样品。以抗坏血酸作为阳性对照,通过考查各部位还原Fe3+的能力、清除二苯基苦基苯肼自由基(DPPH.)和羟基自由基(.OH)的能力、抑制油脂氧化的能力,来评价菥蓂水提物不同部位的抗氧化能力。结果表明:菥蓂的各部位均具有一定的抗氧化能力,但抗氧化活性总体上弱于抗坏血酸和BHT。大孔树脂分离得到的30%乙醇洗脱部位(VII)和乙酸乙酯萃取部位(II)抗氧化能力明显强于菥蓂水提物,稍弱于抗坏血酸。得到结论为萃取、大孔树脂分离纯化等步骤能富集更多的抗氧化成分。
In vitro anti-oxidative activity of the extract from Scutellaria barbata and the effective active site were screened. Through the organic solvent extraction of water extract of Dodder, D101 macroporous resin was separated and purified to prepare samples of 8 parts. Ascorbic acid was used as a positive control to evaluate the ability of various sites to reduce Fe3 +, the ability to scavenge diphenyl picrylhydrazyl free radical (DPPH.) And hydroxyl radical (.OH), and the ability to inhibit lipid oxidation Antioxidant capacity of different parts of water extract. The results showed that: all parts of 菥 蓂 all have some antioxidant capacity, but the overall antioxidant activity is weaker than ascorbic acid and BHT. The anti-oxidative ability of 30% ethanol elution fraction (VII) and ethyl acetate extraction fraction (II) obtained from macroporous resin was significantly stronger than that of water extract of Scutellariae then slightly weaker than that of ascorbic acid. It is concluded that extraction, macroporous resin separation and purification steps can enrich more antioxidant components.