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采用激光拉曼光谱内标法对不同采收期连翘叶中连翘苷的含量进行测定。分别选取连翘苷甲醇溶液的拉曼光谱中连翘苷呋喃环上C-H对称伸缩振动(2844 cm-1处强峰)和溶剂甲醇中CH3-O反对称伸缩振动(2975 cm-1)作为定量峰和内标参比峰。以两拉曼峰峰强的比值组成相对强度,绘制标准曲线,并对线性和加样回收率进行考察。在1.1×10-5mol.L-1~3.5×10-4mol.L-1(5.9 mg.mL-1~189.1 mg.mL-1)范围内,连翘苷的浓度与相对峰强的线性关系良好,r=0.9980,检出限为5.2×10-6mol.L-1.通过精密度、重复性和加样回收率测定,结果证明该法具有良好的精密度、重复性和准确性。对不同采收期的连翘叶样品进行了测定,结果表明该法简便快速、高效灵敏,可用于其含量测定。拉曼光谱内标法用于连翘苷的定量分析具有操作简便和无需添加其它试剂等优点,可以用于药物的含量测定。
The contents of forsythin in Forsythia suspensa collected from different harvest periods were determined by laser Raman spectroscopy. The symmetric stretching vibration of forsythin furan ring (strong peak at 2844 cm-1) and the anti-symmetric stretching vibration of CH3-O (2975 cm-1) in methanol solvent were selected as the quantitative Peak and internal reference peak. The relative intensities of the two Raman peak intensities were calculated, the standard curve was plotted, and the linearity and sample recovery were investigated. The linear relationship between the concentration of forsythin and the relative peak intensity in the range of 1.1 × 10-5mol.L-1 ~ 3.5 × 10-4mol.L-1 (5.9 mg.mL-1 ~ 189.1 mg.mL-1) Good, r = 0.9980, the detection limit of 5.2 × 10-6mol.L-1.The results of precision, repeatability and sample recovery showed that the method has good precision, repeatability and accuracy. The results showed that this method was simple, rapid and sensitive, and could be used for the determination of its content. Raman spectroscopy internal standard method for the quantitative analysis of forsythin has the advantages of easy operation and without the need to add other reagents, can be used for drug content determination.