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目的:探讨五指毛桃水提物对二甲基甲酰胺(DMF)所致小鼠急性肝损伤的保护作用。方法:雌性C57BL/6小鼠25只,雄性ICR小鼠35只,各随机分成5组:正常对照组、DMF组、五指毛桃低、中、高剂量组。五指毛桃各组每天分别灌胃给予五指毛桃水提物100、200、300 g生药/kg,共5 d,第4 d给药30 m in后,除正常对照组外各组小鼠腹腔注射DMF造模。染毒48 h后,测定血清中丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)和乳酸脱氢酶(LDH)的活性,并以光镜观察肝脏组织病理变化。结果:C57BL/6和ICR小鼠各剂量组血清中ALT、AST和LDH活性与模型组比较显著降低,肝脏组织病理损伤明显减轻。结论:五指毛桃对DMF引起的小鼠急性肝损伤具有明显的保护作用。
Objective: To investigate the protective effect of aqueous extracts of five-fingered peach on dimethylformamide (DMF)-induced acute liver injury in mice. Methods: Twenty-five female C57BL/6 mice and 35 male ICR mice were randomly divided into five groups: normal control group, DMF group, and low-, medium-, and high-dose group of five-fingered peaches. Each group of five-fingered peaches was given intragastric administration of 100, 200, and 300 g crude drug/kg each for 5 days, respectively, for 5 days. After 4 days of 30-minute infusion, mice in each group were injected with DMF in the abdominal cavity except for the normal control group. Modeling. After 48 hours of exposure, the activity of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in serum was measured, and the histopathological changes of the liver were observed by light microscopy. Results: The serum levels of ALT, AST and LDH in C57BL/6 and ICR mice were significantly lower than those in the model group, and the liver tissue pathology was significantly reduced. Conclusion: Five-fingered peach has obvious protective effect on DMF-induced acute liver injury in mice.