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以包括22份野生资源的5个耧斗菜物种为试材,采用AFLP分子标记方法,分析了中国耧斗菜野生资源的遗传变异和遗传多样性,为系统鉴定耧斗菜野生资源奠定基础。结果表明:10对引物共扩增出293个多态性位点,多态性位点比率为62.5%。Shannon多样性和He值分析结果表明,5个物种的遗传多样性大小顺序为无距耧斗菜>耧斗菜>尖萼耧斗菜>直距耧斗菜>华北耧斗菜。遗传距离分析结果表明,耧斗菜与尖萼耧斗菜遗传距离最小,尖萼耧斗菜与直距耧斗菜遗传距离最大。
Five wild Brassica campestris species including 22 wild resources were used as experimental materials. AFLP molecular markers were used to analyze the genetic variation and genetic diversity of wild Brachyura camaum compared with those of wild Brassica campestris. The results showed that a total of 293 polymorphic loci were amplified with 10 pairs of primers, and the percentage of polymorphic loci was 62.5%. Shannon diversity and He value analysis showed that the order of genetic diversity among the five species was as follows: Brassica campestris> Caulis carambola> Calyx cauliflower> Cauliflower caulis> Caulis cauliflower. The result of genetic distance analysis showed that the genetic distance between Cabernet Sauvignon and Cephalopoda was the smallest, and the genetic distance between Cephalotaxus and Cabbage was the largest.