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以18个豌豆栽培品种及野生种材料为DNA样品来源,采用7个10碱基随机引物进行PCR扩增。基因组DNA的多态性,利用RAPD标记鉴定其品种间及品种内的遗传变异性。7个引物共扩增出66位点,其中多态性位点56个,占84.8%。每个PCR扩增产物分别以0和1记录存在与否。扩增产物间成对比较产生非相似性矩阵(Jaccard相似系数表),此矩阵用于构建遗传相似性的树状系图谱(遗传树)。通过遗传树的构建将10个P.sativum与8个P.fulvum群居为截然不同的两大组。
Eighteen pea cultivars and wild species were used as DNA samples. Seven 10-base random primers were used for PCR amplification. Genomic DNA polymorphism, the use of RAPD markers identified within the varieties and within the genetic variability. Seven primers amplified a total of 66 loci, of which 56 polymorphic loci, accounting for 84.8%. Each PCR amplification product was recorded as 0 and 1, respectively. Pairwise comparisons of amplified products result in a non-similarity matrix (Jaccard similarity coefficient table), which is used to construct a dendrogram (genetic tree) of genetic similarity. Through the construction of the genetic tree 10 P. sativum with 8 P. Fulvum is divided into two distinct groups.