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目的 探索杀灭肿瘤细胞而最大程度保存神经干再生能力的放射剂量 ,为肿瘤侵犯神经干时保存肢体提供理论依据。方法 35只大白鼠随机分 5组 ,切取大白鼠右侧坐骨神经中段约 10mm ,A组切断后原位再植 ;B、C、D、E组分别以 4 0 0 0、6 0 0 0、80 0 0、10 0 0 0cGy高能X线离体照射后原位再植。定期观察动物受试肢体功能恢复情况。12周后测定各组大白鼠坐骨神经指数、电生理指标 ,并在光镜电镜下观察实验坐骨神经节段形态学改变。结果 坐骨神经指数、神经传导速度及在光镜下轴索数均表明 :A组与C、D、E组之间有显著性差异 (P <0 .0 1) ;B组与C、D、E组之间有显著性差异 (P <0 .0 1) ;A组与B组之间无显著性差异 (P >0 .0 5 )。C、D、E组之间无显著性差异 (P>0 .0 5 )。结论 4 0 0 0cGy高能X线照射可以作为杀灭肿瘤细胞而最大程度保存神经干再生能力的理想放射剂量
OBJECTIVE: To explore the radiation dose for killing tumor cells and preserving nerve regeneration capacity to the maximum extent, and to provide a theoretical basis for preserving limbs when tumors encroach on the neural stem. Methods Thirty-five rats were randomly divided into five groups. The middle part of the right sciatic nerve of the rats was excised for about 10 mm. The rats in group A were replanted in situ after cutting off. The rats in groups B, C, D and E were treated with 40 000, 0 0,10 0 0 0cGy high energy X-ray irradiation in situ replantation. Regular observation of animal limb function recovery. After 12 weeks, sciatic nerve index and electrophysiological indexes of rats in each group were determined. Morphological changes of sciatic nerve segments were observed under light microscope. Results Sciatic nerve index, nerve conduction velocity and axonal number under light microscope showed that there was a significant difference (P <0.01) between group A and groups C, D and E; group B and C, D and E There was significant difference between the two groups (P <0.01). There was no significant difference between group A and group B (P> 0.05). There was no significant difference between C, D and E groups (P> 0.05). Conclusion 4 0 0cGy high-energy X-ray irradiation can be used as the ideal radiation dose for killing tumor cells and preserving the regenerative capacity of neural stems to a maximum extent