【摘 要】
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目的 探讨脂肪来源基质细胞对超长皮瓣成活的影响.方法 利用胶原酶消化SD大鼠腹股沟区的脂肪,经过分离培养传代,将获得的大量脂肪基质细胞行DiI染色,并在SD大鼠背部形成超比
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目的 探讨脂肪来源基质细胞对超长皮瓣成活的影响.方法 利用胶原酶消化SD大鼠腹股沟区的脂肪,经过分离培养传代,将获得的大量脂肪基质细胞行DiI染色,并在SD大鼠背部形成超比例任意皮瓣模型;将DiI染色后的脂肪基质细胞注射到皮瓣下治疗,并与生理盐水治疗组作对比;观察皮瓣大体情况,计算皮瓣成活率;观察病理切片毛细血管密度,荧光显微镜检测脂肪基质细胞在皮瓣内的分布和存活状况.结果 术后第10天,皮瓣成活与坏死部分界线清楚,脂肪基质细胞组皮瓣成活率明显高于生理盐水组.脂肪基质细胞组和生理盐水组的皮瓣存活率分别为(67.4±5.1)％和(52.5±6.5)％,P＜0.05;毛细血管密度分别为(57.1±4.7)/mm2和(28.7±2.8)/mm2,P＜0.05;脂肪基质细胞注射入SD大鼠皮瓣后,脂肪基质细胞存活并参与新生血管的形成.结论 脂肪基质细胞可明显增加超比例任意皮瓣成活面积,其在临床治疗中有潜在的应用和研究价值.
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