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从魔芋根际土壤中分离得到一株具有强烈抑菌活性,并具有较广抑菌谱的革兰氏阳性生防细菌菌株C3.经形态特征、常规生理生化特征和Biolog鉴定,发现该菌株与解淀粉芽孢杆菌Bacillus amyloliquefaciens特征很相似.采用细菌通用引物27f和1 492r扩增16SrDNA基因片段,得到1 105bp DNA片段,其序列与已报道的解淀粉芽孢杆菌FZB42的同源性高达100%,故将生防细菌菌株C3鉴定为解淀粉芽孢杆菌B.amyloliquefaciens.田间防效测试结果显示,在对照病情指数为41.20的情况下,生防细菌C3对魔芋细菌性软腐病的控制效果达到了57.76%.
A Gram-positive biocontrol bacterial strain C3 with strong antimicrobial activity and wide spectrum of inhibition was isolated from rhizosphere soil of konjac.The morphological, physiological and biochemical characteristics and Biolog identification showed that the strain was associated with The characteristics of Bacillus amyloliquefaciens were similar.Using common bacterial primers 27f and 1 492r to amplify the 16SrDNA gene fragment, a 105bp DNA fragment was obtained, whose sequence had 100% homology with the previously reported B. amyloliquefaciens FZB42 The biocontrol bacterial strain C3 was identified as Bacillus amyloliquefaciens B. Field control test showed that biocontrol bacterium C3 had a control effect on bacterial soft rot of konjac when the control index was 41.20, reaching 57.76 %.