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采用280nm和260nm紫外吸收、双缩脲法、甲醛法、凯氏定氮法以及640nm可见光吸收法对小梅鱼酶法水解过程中蛋白质、核酸、α氨基氮和总氮含量以及浊度变化等指标进行了过程监控。实验结果表明可以用280nm紫外吸收法代替凯氏定氮法分析小梅鱼水解过程中清液的总氛含量变化，过程中280nm紫外吸收值的变化呈规律性递增。水解产物中总氮含量与280nm紫外吸收值的关系为Y＝2．016X-0．04。260nm紫外吸收值的递增可说明鱼蛋白质水解的过程伴随着核酸的释放。双缩脲反应测定值在过程中递减，说明清液中有大分子可溶蛋白或长链肽被降解。甲醛法测定α氨基氮值与鲜味的增加成正比。
Using 280nm and 260nm ultraviolet absorption, biuret method, formaldehyde method, Kjeldahl method and 640nm visible light absorption method, the contents of protein, nucleic acid, α-amino nitrogen and total nitrogen, Process monitoring was conducted. The experimental results show that 280 nm ultraviolet absorption method can be used instead of Kjeldahl method to analyze the change of the total content of the supernatant liquid in the process of the hydrolysis. The change of the 280 nm UV absorbance shows a regular increase. The relationship between total nitrogen content in hydrolyzate and 280nm UV absorbance is Y = 2.016X-0.04.260nm The increase of UV absorbance shows that the process of fish protein hydrolysis is accompanied by the release of nucleic acid. Determination of biuret reaction decreased in the process, indicating that the soluble protein in the supernatant or long-chain peptide degradation. Formaldehyde determination of α-amino nitrogen value is proportional to the increase of umami.