论文部分内容阅读
目的 探讨微小RNA-335-5p(miR-335-5p)在骨关节炎患者外周血单个核细胞(PBMC)中的表达及其意义.方法 收集50例骨关节炎患者及30例健康志愿者的外周抗凝血,分离PBMC,实时定量PCR检测miR-335-5p表达水平.分析miR-335-5p与骨关节炎患者的年龄、BMI及Kellgren-Lawrence X线分级之间的相关性.结果 骨关节炎患者PBMC中miR-335-5p表达水平低于健康对照组(0.00090±0.00008 vs. 0.00278±0.00025,P<0.001),骨关节炎患者PBMC中miR-335-5p表达水平与其年龄(r=-0.763,P<0.001)、BMI(r=-0.440,P0.05).结论 miR-335-5p在骨关节炎患者PBMC中表达下调,与年龄、BMI呈负相关,提示其可能是骨关节炎的保护性基因.“,”Objective To investigate the expression and significance of microRNA-335-5p (miR-335-5p) in the peripheral blood mononuclear cells (PBMCs) of patients with osteoarthritis. Methods The peripheral blood samples were collected from 50 osteoarthritis patients and 30 healthy controls. The PBMCs were isolated from the blood samples. The expression level of miR-335-5p was detected using quantitative real-time reverse transcription PCR (qRT-PCR). The correlation between miR-335-5p and age, body mass index (BMI) and Kellgren-Lawrence classification for knee osteoarthritis was analyzed. Results The expression of miR-335-5p in the PBMCs of osteoarthritis patients was significantly down-regulated compared with that in the healthy controls (0.00090±0.00008 vs. 0.00278±0.00025, P<0.001). In the PBMCs of osteoarthritis patients, the expression of miR-335-5p was negatively correlated with the age (r=-0.763, P<0.001) and BMI (r=-0.440, P0.05). Conclusions The expression of miR-335-5p is significantly down-regulated in the PBMCs of osteoarthritis patients. Moreover, it is negatively correlated with age and BMI, suggesting that miR-335-5p might be a protective gene for osteoarthritis.