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目的：探讨重组人碱性成纤维细胞生长因子（ｒｈｂＦＧＦ） 对人羊水细胞及其它细胞的影响。方法：在低血清培养基培养条件下进行人羊水细胞及Ｂａｌｂ／ｃ 小鼠巨噬细胞和３Ｔ３ 成纤维细胞，采用四甲基偶氮唑盐（ ＭＴＴ） 比色法和倒置显微镜观察ｒｈｂＦＧＦ 对人羊水细胞存活的影响。结果：人羊水细胞、Ｂａｌｂ／ｃ 小鼠巨噬细胞和３Ｔ３ 成纤维细胞在０－４ ％ 胎牛血清培养基培养条件下，不同浓度的ｒｈｂＦＧＦ 对羊水细胞的作用存在相关性。结论：ｒｈｂＦＧＦ 能延长羊水细胞、Ｂａｌｂ／ｃ小鼠巨噬细胞和３Ｔ３ 成纤维细胞的存活时间
Objective: To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on human amniotic fluid cells and other cells. Methods: Human amniotic fluid cells and Balb / c mouse macrophages and 3T3 fibroblasts were cultured in low serum medium. MTT assay and inverted microscope were used to observe the effect of rhbFGF on human Amniotic fluid cell survival effects. Results: The human amniotic fluid cells, Balb / c mouse macrophages and 3T3 fibroblasts cultured in 0-4% fetal bovine serum medium had different concentrations of rhbFGF on the role of amniotic fluid cells. Conclusion: rhbFGF can prolong the survival time of amniotic fluid cells, Balb / c mouse macrophages and 3T3 fibroblasts