论文部分内容阅读
链霉素在使用中可引起过敏反应,其发生率仅次于青霉素。我国链霉素过敏休克反应亦屡见不鲜,但对链霉素抗原结构的研究,国内外文献尚未见报道。我们用纯链霉素、纯双氢链霉素与福氏完全佐剂免疫家兔,用链霉素致敏血球的间接血凝试验,分别测出链霉素或双氢链霉素抗体,其最高效价为1:1024。又利用已知化学结构半抗原的间接血凝抑制试验,探讨链霉素抗原结构。试验结果,链霉素1.56μg可抑制间接血凝反应;而链霉素分子中链霉胍、链霉二糖胺及链霉糖碱水解物麦芽酚等间接血凝抑制量为500～1000μg,表明已丧失链霉素的抗原结构。链霉素结构中醛基的氧化物链
Streptomycin in use can cause allergic reactions, the incidence of penicillin. Streptomycin allergic shock response is also common in our country, but the study of streptomycin antigen structure has not been reported at home and abroad. We use pure streptomycin, pure dihydrostreptomycin and Freund’s complete adjuvant immune rabbits, with streptomycin-sensitized blood cells of indirect hemagglutination test were detected streptomycin or dihydrostrepton antibody, Its highest potency is 1: 1024. Also use the known chemical structure of hapten indirect hemagglutination inhibition test to explore the streptomycin antigen structure. Test results, streptomycin 1.56μg can inhibit the indirect hemagglutination reaction; and streptomycin molecules Streptozotocin, streptobisosamine and streptolysin hydrolyzate, maltol and other indirect hemagglutination inhibition of 500 ~ 1000μg, Indicating that the antigenic structure of streptomycin has been lost. Oxidative chain of aldehyde groups in streptomycin structure