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目的采用两种不同方法建立人胃癌裸鼠皮下移植瘤模型。方法人胃癌细胞悬液直接注射法:将处于对数生长期的人胃癌细胞悬液接种于裸鼠皮下;胃癌裸鼠瘤转瘤法:将胃癌细胞悬液接种入裸鼠,待肿瘤生长直径在8 mm左右时,处死裸鼠并取其肿瘤组织边缘新鲜的组织,接种于裸鼠皮下,形成皮下移植瘤。观察并比较两种方法所建立的模型肿瘤移植成功率及瘤体生长速度,实验结束后MSP法检测移植瘤组织中Reprimo基因的甲基化水平并采用免疫组织化学法检测移植瘤的Ki-67和CD31分子表达。结果细胞悬液直接注射组的瘤体成瘤速度较瘤转瘤组慢(P<0.05);瘤转瘤组的Reprimo基因甲基化水平及Ki-67和CD31的表达均高于细胞悬液直接注射组(P<0.05)。结论胃癌裸鼠皮下瘤转瘤法的瘤体生长状况优于胃癌细胞悬液直接注射法。
Objective To establish a human gastric cancer xenograft model in nude mice by two different methods. Methods Human gastric cancer cell suspension direct injection method: in the logarithmic growth phase of human gastric cancer cell suspension inoculated in nude mice subcutaneous; gastric cancer in nude mice tumor method: the gastric cancer cell suspension inoculated into nude mice until the tumor growth diameter At about 8 mm, the nude mice were sacrificed and the fresh tissue on the margin of the tumor tissue was inoculated into the skin of nude mice to form a subcutaneous xenograft tumor. The success rates of tumor transplantation and tumor growth were observed and compared. The MSP assay was used to detect the methylation level of Reprimo gene in the tumor tissue and the Ki-67 And CD31 molecule expression. Results The rate of tumorigenesis in the direct injection group was slower than that in the tumor-bearing group (P <0.05). The methylation level of Reprimo gene and the expression of Ki-67 and CD31 in the tumor-bearing group were higher than those in the cell suspension Direct injection group (P <0.05). Conclusion The growth of tumor in the subcutaneous tumor-bearing tumor of nude mice is better than the direct injection of gastric cancer cell suspension.