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高等植物中花青素合成与芹菜素合成之间存在共同的前体物质。利用非紫色芹菜‘六合黄心芹’和紫色芹菜‘南选六合紫芹’(从‘六合黄心芹’中选择而来)的叶柄为花青素和芹菜素代谢研究材料,利用荧光定量PCR方法检测花青素和芹菜素合成相关基因的表达水平。研究结果表明,‘六合黄心芹’叶柄中未检测到花青素积累,而‘南选六合紫芹’叶柄中的花青素含量呈现较高水平(0.0523 mg·g~(-1)FW)。‘六合黄心芹’叶柄芹菜素含量(0.0172 mg·g~(-1) FW)显著高于‘南选六合紫芹’(0.0124 mg·g~(-1) FW)。荧光定量PCR结果表明,除了AgFNS之外,其余花青素和芹菜素代谢相关基因(AgPAL、AgC4H、AgCHS、AgCHI、AgFNS、AgF3H、AgF3’H、AgDFR、AgANS和Ag3GT)在‘南选六合紫芹’叶柄中的表达量显著或者极显著高于‘六合黄心芹’;黄酮合成酶基因AgFNS在‘六合黄心芹’叶柄中的表达量为‘南选六合紫芹’的11.69倍。
Common precursors exist between anthocyanin synthesis and apigenin synthesis in higher plants. The petioles of non-purple celery “Liuhe Huangxinqin” and purple celery “Nanxiao Liuhe Qinqin” (selected from ’Liuhe Huangxinqin’) were anthocyanin and apigenin metabolic materials, and the detection of cyanine by fluorescence quantitative PCR Su and apigenin synthesis related gene expression levels. The results showed that no anthocyanin accumulation was detected in the petiole of ’Lihezi’, while that of the ’south selection’ was higher (0.0523 mg · g -1 FW). The content of apigenin (0.0172 mg · g ~ (-1) FW) in ’Liuhe Huangxinqin’ was significantly higher than that in the ’Southern selection’ (0.0124 mg · g -1 FW). Fluorescent quantitative PCR results showed that in addition to AgFNS, anthocyanin and apigenin metabolism related genes (AgPAL, AgC4H, AgCHS, AgCHI, AgFNS, AgF3H, AgF3’H, AgDFR, AgANS and Ag3GT) The expression level of AgFNS in petiole was significantly higher or significantly higher than that of ’Liuhe Huangxinqin’. The expression level of AgFNS was 11.69 folds of that of ’South Qinlian Ziqin’.