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[目的]观察微囊蛋白1(caveolin-1)抗体对汽油机尾气所致肺损伤的保护作用。[方法]雄性健康SD大鼠42只,随机分为正常对照组6只、染毒对照组6只、干预组30只(分1、2、4、8、12周亚组,每亚组6只)。正常对照组不作任何处理,正常喂饲;染毒对照组以汽油机尾气持续染毒12周,每天染毒2h;干预组各亚组在开始染毒后的前2周让实验动物吸入超声雾化的caveolin-1多克隆抗体,并分别用汽油机尾气染毒1、2、4、8、12周。各组大鼠在最后一次染毒24h后处死,对肺组织进行常规病理形态学观察,用免疫组织化学技术检测肺部caveolin-1和转化生长因子β1(TGF-β1)表达变化。[结果]染毒对照组大鼠体重明显较正常对照组轻,干预组体重介于正常对照组与染毒对照组之间。干预组炎症反应较染毒对照组明显减轻,纤维组织增生减少。正常对照组可以检测到较弱的caveolin-1表达;染毒对照组则几乎检测不到;干预组表达先增强后减低,但实验后期仍较染毒对照组略高。TGF-β1表达在正常对照组最低;染毒对照组最高;干预组逐渐升高,但较染毒对照组明显减弱。[结论]早期使用caveolin-1多克隆抗体可部分减轻汽油机尾气造成的肺部损伤。
[Objective] To observe the protective effect of caveolin-1 antibody on lung injury induced by gasoline engine exhaust. [Methods] Forty-two male SD rats were randomly divided into normal control group (n = 6), exposure control group (n = 6) and intervention group (n = 30) only). The normal control group was given no treatment and the rats were fed normally. The control group was exposed to gasoline engine exhaust gas for 12 weeks and exposed to daily for 2 hours. In the first two weeks after exposure, the experimental group received ultrasonic atomization Of caveolin-1 polyclonal antibody, respectively, and gasoline tail gas exposure 1,2,4,8,12 weeks. The rats in each group were sacrificed 24h after the last exposure and the histopathology of the lungs was observed. The expression of caveolin-1 and transforming growth factor-β1 (TGF-β1) in the lungs were detected by immunohistochemistry. [Result] The body weight of the control group was obviously lighter than that of the normal control group, and the body weight of the intervention group was between the normal control group and the exposure control group. Compared with the control group, the inflammatory response in the intervention group was significantly reduced, and the proliferation of fibrous tissue decreased. The normal control group could detect the weaker expression of caveolin-1; the control group was almost undetectable; the expression of intervention group first increased and then decreased, but the latter was still slightly higher than the control group. The expression of TGF-β1 in the normal control group was the lowest; the exposure control group was the highest; the intervention group was gradually increased, but was significantly weaker than the control group. [Conclusion] The early use of caveolin-1 polyclonal antibody can partially reduce the lung injury caused by gasoline engine exhaust.