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以羟基化多壁碳纳米管(MWNT-OH)为引发剂,通过原位负离子开环聚合制备了生物相容性多羟基超支化聚缩水甘油接枝的碳纳米管(MWNT-HPG),利用酯化反应将荧光分子罗丹明6B标记于聚合物上,然后聚合物上的羟基和丁二酸酐反应将其转化为羧基.用TGA、FTIR、TEM、SEM等手段对产物进行了表征.用靶向表皮生长因子受体(EGFR)的小分子多肽D4修饰了所得的荧光功能化碳纳米管,并将其做为受体介导靶向肿瘤细胞的纳米载体,通过噻唑蓝(MTT)比色法评价功能化碳纳米管作为载体的安全性.用荧光显微镜观察其与人肺腺癌细胞SPCAI细胞的结合状态.结果证明了其有希望成为受体介导靶向肿瘤系统的纳米载体.
Biocompatible polyhydroxy hyperbranched polyglycidyl-grafted carbon nanotubes (MWNT-HPG) were prepared by in-situ anion ring-opening polymerization using hydroxylated multiwalled carbon nanotubes (MWNT-OH) as initiators. The esterification reaction labeled the fluorescent molecule rhodamine 6B on the polymer, and then the hydroxyl group on the polymer reacted with succinic anhydride to convert it into carboxyl group.The product was characterized by TGA, FTIR, TEM, SEM etc. With the target The resulting fluorescently functionalized carbon nanotubes were modified with small molecule peptide D4 of epidermal growth factor receptor (EGFR) and used as a receptor-mediated nanocarrier targeting tumor cells. MTT colorimetric assay Method was used to assess the safety of functionalized carbon nanotubes as a carrier.The binding status of the functionalized carbon nanotubes to the SPCAI cells was observed under a fluorescence microscope.The results demonstrate that it is promising as a nanocarrier for the receptor mediated tumor targeting system.