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本文利用原位PCR技术和荧光原位杂交技术,对巴西橡胶树4个环锌指蛋白基因(HbRZF)在染色体的位置进行了物理定位分析。结果表明:用原位PCR技术检测到的HbRZF1和HbRZF3扩增信号分别定位于巴西橡胶热研7-33-97品种的第6号和第5号染色体长臂上,信号距着丝粒平均百分距离分别是45.76±3.18和66.33±0.75,信号检出率分别为28.79%和36.70%。用荧光原位杂交技术检测到的HbRZF2和HbRZF4探针信号分别位于第3号和7号染色体长臂上,信号距着丝粒平均百分距离分别为22.25±1.02和40.64±1.44,两种信号同时检出的机率为12.18%。本研究还对这些基因与其他定位的基因之间的连锁关系进行了讨论。
In this study, we used in situ PCR and fluorescence in situ hybridization to analyze the location of four ring zinc finger protein genes (HbRZF) on the chromosomes. The results showed that the amplified signals of HbRZF1 and HbRZF3 detected by in situ PCR were located on the long arms of chromosome 6 and 5 of PyroBan 7-33-97, The distances were 45.76 ± 3.18 and 66.33 ± 0.75 respectively, and the signal detection rates were 28.79% and 36.70% respectively. HbRZF2 and HbRZF4 probe signals detected by fluorescence in situ hybridization were located on the long arms of chromosomes 3 and 7, respectively. The average distance from the centromeres to the signal was 22.25 ± 1.02 and 40.64 ± 1.44, respectively. Both signals At the same time the probability of detection was 12.18%. This study also discusses the linkage between these genes and other genes that are located.