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目的分析衡阳市2009-2011年度流感病毒流行情况,了解病毒毒株的型别及变化特点,为预防和控制流感提供科学依据。方法采集流感样病例(ILI)的鼻咽拭子标本,采用狗肾细胞(MDCK)进行病毒分离和(或)RT-PCR方法检测病毒核酸,分离到的毒株采用血凝抑制实验(HI)进行流感病毒型别鉴定。结果 2009年3月-2011年2月共采集2 682份ILI咽拭子标本,采用病毒分离887份,分离到毒株116株,分离阳性率13.08%,其中季节性A(H1N1)亚型31株,季节性A(H3N2)亚型35株,B-victoria系36株,B-yamagata系6株,新甲型H1N1 8株;采用核酸检测2 143份,核酸阳性737份,阳性率34.39%,其中新甲型H1N1 543份,季节性A型146份,季节性B型48份。结论 2009年3月-2011年2月流感毒株各型别阶段性交替形成优势株。全年皆有流感样病例,夏季高峰较明显。2009年是流感大流行年,9月新甲型H1N1的流行成为主导,并在11-12月份达到流行高峰。为更好防控流感,及时掌握流行趋势,仍需加强监测。
Objective To analyze the epidemic situation of influenza virus in Hengyang from 2009 to 2011, understand the type and variation characteristics of virus strains and provide a scientific basis for the prevention and control of influenza. Methods Nasopharyngeal swab samples of influenza-like illness (ILI) were collected. The virus nucleic acid was detected by MDCK and / or RT-PCR. The isolated HI strains were determined by hemagglutination inhibition (HI) Conduct influenza virus type identification. Results A total of 2 682 ILI throat swabs were collected from March 2009 to February 2011. 887 strains of ILI were isolated and 116 strains were isolated. The positive rate was 13.08%. The seasonal A (H1N1) subtype 31 35 strains of seasonal A (H3N2) subtype, 36 strains of B-victoria strain, 6 strains of B-yamagata strain and 8 strains of new H1N1 virus were detected; 2 143 were detected by nucleic acid, 737 were positive for nucleic acid, the positive rate was 34.39% , Of which 543 were new type A H1N1, 146 were seasonal type A and 48 were type B seasonal. Conclusion From March 2009 to February 2011, all the strains of flu strains formed predominant strains alternately. Influenza-like cases all year round, summer peak is more obvious. The year 2009 was a year of pandemic influenza. In September, the popularity of new H1N1 became the dominant epidemic and reached its peak in November-December. In order to better control the flu and keep abreast of the epidemic, we still need to strengthen monitoring.