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目的 :探讨三参滋胃饮对慢性萎缩性胃炎 (CAG)大鼠胃黏膜 G细胞和 D细胞的作用及其机制。方法 :采用综合法 (主动免疫、寒凉胆汁及热水灌胃等 )复制 CAG大鼠模型 ,应用大、小剂量三参滋胃饮及对照药物维酶素 (WMS)连续给药 5 0 d,观察给药前后各组胃黏膜病理改变、免疫组化染色后 G细胞及 D细胞的形态和数量变化。结果 :萎缩性胃炎模型鼠 G细胞和 D细胞数量明显减少 (P<0 .0 1 ) ,且两种细胞均有脱颗粒现象 ;G/D比值下降 ,表明在萎缩发生期间 G细胞下降速度较 D细胞快 ,即 G细胞对萎缩病变反映更为敏感。给药结束后三参滋胃饮组 G、D细胞数量均明显增多 ,最终使 G/D比值恢复至正常组水平 ,且与 WMS组比较差异有显著性(P<0 .0 5 )。大剂量组病理改变的恢复情况优于小剂量组。结论 :三参滋胃饮可能通过改善胃黏膜炎症反应促进腺体及内 (旁 )分泌细胞再生 ,通过改善胃肠激素对胃功能的调节作用而发挥治疗作用。
Objective: To investigate the effect and mechanism of Sanshen Ziwei Decoction on gastric mucosal G cells and D cells in chronic atrophic gastritis (CAG) rats. METHODS: The CAG rat model was duplicated by a comprehensive method (active immunity, cold bile and hot water gavage, etc.) and continuous administration of large and small doses of Sanshen Ziwei Decoction and control drug retinoid (WMS) for 50 days. Observe the change of morphology and quantity of G cells and D cells after immunohistochemical staining. RESULTS: The number of G cells and D cells in the atrophic gastritis model mice was significantly reduced (P < 0.01), and both cells had degranulation; the G/D ratio decreased, indicating that the rate of G cell decline during atrophy D cells are faster, that is, G cells are more sensitive to atrophic lesions. After the administration, the number of G and D cells in the Sanshen Ziwei Decoction group increased significantly, eventually returning the G/D ratio to the normal group, and there was a significant difference compared with the WMS group (P<0.05). The recovery of pathological changes in the high-dose group was better than that in the low-dose group. Conclusion : Sanshen Ziwei Decoction may promote the regeneration of glandular and intra-paracellular secretion cells by improving the inflammatory response of gastric mucosa, and play a therapeutic role by improving the regulatory effect of gastrointestinal hormones on gastric function.