为使体外培养的角朊细胞在近似体内状况下生长,本实验将人体表皮角朊细胞置于塑料培养环内的胶原基质上进行空气—液体交界面培养。培养14d后,将培养物冰冻切片,行普通细胞染色和免疫细胞化学检查,结果显示,有多层细胞形成,且在上层细胞中出现filaggrin。提示此培养技术可用于研究人体表皮细胞的生长和分化。 In order to make keratinocytes cultured in vitro grow in an approximately in vivo condition, human epidermal keratinocytes are placed on a collagen matrix in a plastic culture ring for air-liquid interface culture. After culturing for 14 days, the culture was frozen and sectioned. Normal cell staining and immunocytochemistry were performed. The results showed that many layers of cells formed and filaggrin appeared in the upper layer cells. Tip This culture technique can be used to study human epidermal cell growth and differentiation.