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目的:建立一种离子色谱梯度淋洗抑制电导法快速测定尿液中草酸根、磷酸根及枸橼酸根离子浓度。方法:采集新鲜的尿样,经高速离心过0.2μm的微孔滤膜、On-Guard H小柱及C18预处理小柱后直接进样。选用Dionex IonPac AG23(50mm×4mm)阴离子保护柱和Dionex IonPac AS23(250mm×4mm)阴离子分析柱对样品进行分离,氢氧化钾溶液梯度淋洗,自再生抑制电导检测器检测。结果:线性范围为0.5～50μg·mL-1,r≥0.9998;日内精密度RSD≤3.6%,日间精密度RSD≤6.9%;样品加标平均回收率为96.0%～100.2%。结论:该方法操作简便、灵敏,选择性高,适用于常规样品分析。
OBJECTIVE: To establish a ion chromatography gradient elution inhibition conductivity method for rapid determination of urinary oxalate, phosphate and citrate ion concentrations. Methods: Fresh urine samples were collected and directly injected through a 0.2 μm microporous membrane filter, On-Guard H cartridge and C18 pretreatment cartridge. Samples were separated using a Dionex IonPac AG23 (50 mm × 4 mm) anion guard column and a Dionex IonPac AS23 (250 mm × 4 mm) anion analytical column, and a potassium hydroxide solution gradient elution was performed from a regenerative inhibition conductivity detector. Results: The linear range was 0.5-50 μg · mL-1, r≥0.9998. The intra-day precision RSD was less than or equal to 3.6% and the intra-day precision RSD was less than or equal to 6.9%. The average spiked recoveries were 96.0% -100.2%. Conclusion: The method is simple, sensitive and selective, suitable for routine sample analysis.