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目的:探讨灵巴菌质油对人肺癌细胞A549成瘤能力及伪足形成的效应。方法:灵巴菌质油25,50,100 mg·L-1分别作用A549细胞20 d和24 h,通过软琼脂克隆形成法和PI单染法观察灵巴菌质油对A549细胞的成瘤能力和生长周期的影响;灵巴菌质油100 mg·L-1作用A549细胞24 h,通过免疫荧光染色法观察细胞骨架的影响。结果:灵巴菌质油能够抑制细胞克隆的形成,低、中、高剂量组作用A549细胞20 d后的平均细胞克隆形成数与空白对照组比较均有极显著差异(P<0.01),抑制率与药物浓度呈正相关,并将细胞的生长阻滞在G2期,中、高剂量组G2期细胞比例分别为21.92%和29.94%,与空白对照组12.44%相比均有显著性差异(P<0.05);但灵巴菌质油能改变A549细胞中微丝结构的分布,使其产生伪足。结论:灵巴菌质油具有降低A549细胞克隆形成能力及损伤其细胞骨架的作用,但它亦可能导致瘤细胞的迁移。
Objective: To investigate the effect of Lingbamycin on tumorigenicity and pseudopod formation of human lung cancer cell line A549. Methods: A549 cells were treated with Lingbacao 25, 100 and 100 mg · L-1 for 20 d and 24 h, respectively. The tumorigenicity of Lingbamycin on A549 cells was observed by soft agar cloning and PI staining. The growth of A549 cells was stimulated with 100 mg · L-1 of Lingbamycin oil for 24 h. The cytoskeleton was observed by immunofluorescence staining. RESULTS: Lingbamycin could inhibit the formation of cell clones. The average number of colony forming cells in A549 cells treated with low, medium and high doses of 20 min was significantly different from that of the blank control group (P <0.01) There was a positive correlation between the rate and the drug concentration, and the cell growth was blocked in the G2, G2 and G2 phases of the medium and high dose groups respectively 21.92% and 29.94%, which was significantly different from that of the blank control group (P <0.05). However, the ampicillin oil can change the distribution of microfilament structure in A549 cells to produce pseudopodia. CONCLUSION: Lingbacterium oil can reduce the colony-forming ability and damage the cytoskeleton of A549 cells, but it may also cause the migration of tumor cells.