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目的:建立同时测定敷胸巴布贴中5种大黄蒽醌类成分(芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚)含量的方法。方法:采用Agilent TC-C18(250 mm×4.6 mm,5μm)色谱柱,流动相甲醇-0.4%磷酸溶液(85:15),流速1.0 m L·min-1,柱温30℃,紫外检测波长254 nm。结果:5种蒽醌类成分之间有较好的分离度,芦荟大黄素线性范围0.094 2~0.942μg(r=0.9995),大黄酸线性范围0.036 9~0.369μg(r=0.9996),大黄素线性范围0.048 6~0.486μg(r=0.9999),大黄酚线性范围0.032 4~0.324μg(r=0.9998),大黄素甲醚线性范围0.069 6~0.696μg(r=0.9997),平均加样回收率分别为98.7%,98.3%,96.8%,96.8%,97.4%,RSD分别为0.7%,1.0%,2.1%,1.8%,2.4%。结论:该实验方法精确度高、重复性好,可用于敷胸巴布贴的质量控制。
Objective: To establish a method for the simultaneous determination of five anthraquinone components (aloe-emodin, rhein, emodin, chrysophanol, and physcion) in Radix Paeoniae Barbata. METHODS: The mobile phase consisted of methanol-0.4% phosphoric acid solution (85:15), flow rate 1.0 m L · min-1 and column temperature 30 ℃ with an Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) 254 nm. Results: There was a good resolution between five anthraquinones. The linear range of aloe-emodin was 0.094 2-0.992 μg (r = 0.9995), the linear range of rhein was 0.036 9-0.3699 μg (r = 0.9996) The linear range was 0.048 6 ~ 0.486μg (r = 0.9999), the linear range of chrysophanol was 0.032 4 ~ 0.324μg (r = 0.9998), and the linear range of physcion was 0.069 6 ~ 0.696μg (r = 0.9997) They were 98.7%, 98.3%, 96.8%, 96.8% and 97.4% respectively. The RSDs were 0.7%, 1.0%, 2.1%, 1.8% and 2.4% respectively. Conclusion: The experimental method is accurate, reproducible and can be used for the quality control of Chestnut patch.