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目的 研究新城疫病毒疫苗对小鼠S1 80 肉瘤细胞的作用。方法 采用昆明种小鼠皮下移植性S1 80 肉瘤模型 ,Ⅰ组瘤体内注射PBS液 ,Ⅱ、Ⅲ组瘤体内注射NDV疫苗 (Ⅱ组在接种S1 80 肉瘤前 1周皮下注射NDV疫苗 ) ,分 2次解剖取材后光镜、电镜观察。结果 NDV颗粒附着在瘤细胞表面 ,瘤组织大片坏死 ,坏死周边部有炎细胞浸润 ,并可见凋亡小体。Ⅱ、Ⅲ组瘤体逐渐减小 ,有 2只Ⅱ组小鼠瘤块完全消失 ,生存期延长。其他脏器无明显病理改变。结论 新城疫病毒疫苗对小鼠S1 80 肉瘤细胞有较强的杀伤作用 ,抑制肿瘤的生长 ,安全性大。
Objective To study the effect of Newcastle disease virus vaccine on mouse S180 sarcoma cells. Methods Kunming mice subcutaneously transplanted with S180 sarcoma model, group Ⅰ tumor in vivo injection of PBS solution, Ⅱ, Ⅲ group in vivo injection of NDV vaccine (group Ⅱ inoculated with S180 sarcoma 1 week prior to subcutaneous injection of NDV vaccine), divided into 2 Secondary anatomic material after light and electron microscopy. Results NDV particles attached to the surface of tumor cells, tumor necrosis, inflammatory cell infiltration around the necrosis, and apoptotic bodies can be seen. The tumors in group Ⅱ and Ⅲ decreased gradually, and the tumors in group Ⅱ completely disappeared and the survival time was prolonged. Other organs no significant pathological changes. Conclusion Newcastle disease virus vaccine has a strong killing effect on mouse S180 sarcoma cells and inhibits the growth of the tumor and is safe.