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鲨鱼软骨粉（ＳＣＰ）伍用“４２５”制剂（当归根提取物），使成鲨鱼软骨粉合剂，用以研究感染日本血吸虫３ｗｋ的ＢＡＬＢ／ｃ小鼠经口服用ＳＣＰ合剂后４ｗｋ（相当于感染后７ｗｋ）诱导的抗血吸虫性纤维化效应及其机制。测定实验小鼠肝内血吸虫卵肉芽肿（ＳＥＧ）大小和纤维化程度；用ＥＬＩＳＡ检测小鼠血清抗体ＩｇＧ及其亚类ＩｇＧ１水平（ＯＤ均值）；应用流式细胞仪测定小鼠脾脏Ｔ细胞亚群的细胞均数和比值。实验结果：服用ＳＣＰ合剂（Ⅰ组）和单用ＳＣＰ（Ⅲ组）小鼠的ＳＥＧ大小均显著小于感染对照鼠（Ⅳ组），且前两者肝内血吸虫性纤维化均已消失，而后者显示严重的肝纤维化。单用“４２５”制剂（Ⅱ组）与Ⅰ组和Ⅲ组的血吸虫卵肉芽肿减小率之间无显著性差异，但Ⅱ组仍呈现轻度的肝血吸虫性纤维化。Ⅰ组和Ⅲ组的ＣＤ４＋／ＣＤ８＋比值（１．７０～１．８８）明显高于Ⅱ组（０．９５）；Ⅰ组和Ⅱ组的ＩｇＧ和ＩｇＧ１水平均显著高于Ⅲ组和Ⅳ组。结果表明，服用ＳＣＰ合剂和单用ＳＣＰ小鼠，均能诱导明显的抗肝血吸虫性纤维化效应，若单用“４２５”制剂，则仅显示轻度的抗肝纤维化作用，其机制与细胞免疫密切相关。
Shark cartilage powder (SCP) was used as a shark cartilage powder mixture to study the effects of infection with the “425” preparation (Angelica root extract) on BALB / c mice infected with Schistosoma japonicum at 3 weeks after 4 weeks of oral administration of SCP mixture After 7wk) induced anti-schistosomiasis fibrosis and its mechanism. The size and fibrosis of egg granuloma (SEG) of mice intrahepatic schistosoma were measured. The level of serum IgG and its subclass IgG1 (OD mean) were detected by ELISA. The level of T lymphocyte subsets in mouse spleen was measured by flow cytometry Cellular mean and ratio of cells. The results showed that the SEG sizes of mice in group Ⅰ and group SCP were significantly smaller than those in group Ⅳ (Ⅳ), and the former two had no hepatic schistosome fibrosis Shows severe liver fibrosis. There was no significant difference between the reduction rate of egg granuloma in group 425 and group 425 only (P <0.05), but there was still mild liver schistosome fibrosis in group II. The CD4 + / CD8 + ratio (1.70-1.88) in group Ⅰ and group Ⅲ was significantly higher than that in group Ⅱ (0.95). The levels of IgG and IgG1 in group Ⅰ and group Ⅱ were significantly higher than those in group Ⅲ and group Ⅳ. The results showed that both SCP and SCP mice were able to induce obvious anti-hepatic schistosome fibrosis effects. If only “425” formulation was used, only mild anti-hepatic fibrosis was shown. The mechanism was similar to that of cells Immune is closely related.