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目的:对国内外公开发表的中国原发性肝癌与miR-146a G>C(rs2910164)遗传易感性的相关文献进行Meta分析。方法:检索Pub Med数据库、Cochrane Library数据库、中国学术期刊全文数据库(CNKI)和万方数据库获得文献。检索时限为自建库至2017年3月。采用Stata 12.0软件进行Meta分析,计算各基因型的合并比值比(OR)及95%置信区间(CI)。结果:共纳入9篇病例对照研究,累计病例组3 937例,健康对照组5 025例,研究间存在中等强度的异质性。固定效应的合并OR值及95%CI在显性模型[(GG+GC)/CC]和隐性模型[GG/(GC+CC)]分别为1.26(1.12,1.41)(P=0.00)和1.23(1.12,1.34)(P=0.00),随机效应等位模型(G/C)为1.17(1.10,1.25)(P=0.00)。Meta回归分析发现显性模型样本的地域来源与合并OR值正相关。亚组分析表明,在中东人群和西部人群及采用PCR-RFLP分型时,miR-146a G>C与原发性肝癌的遗传易感性密切相关;而在西南人群和采用非PCR-RFLP(Non-PCR-RFLP)分型时无明显相关性。三个基因型的敏感性分析结果稳定,也没有发表偏倚。结论:miR-146a G>C与原发性肝癌的遗传易感性密切相关,携带G等位的个体有更高的发病风险,该位点基因多态性具有成为原发性肝癌的生物标记物的潜能。
OBJECTIVE: To carry out a meta-analysis of published literature about the genetic susceptibility of Chinese primary liver cancer and miR-146a G> C (rs2910164). Methods: We searched PubMed database, Cochrane Library database, CNKI and Wanfang database for literature. The search period is from self-built library to March 2017. Meta-analysis was performed using Stata 12.0 software to calculate the combined odds ratio (OR) and 95% confidence interval (CI) for each genotype. Results: A total of 9 case-control studies were included, with 3 937 cumulative cases and 5 025 healthy controls. There was moderate heterogeneity among the studies. The combined OR and 95% CI of the fixed effect were 1.26 (1.12, 1.41) (P = 0.00) and 1.23 (1.12,1.34) (P = 0.00). The random effects allele model (G / C) was 1.17 (1.10,1.25) (P = 0.00). Meta-regression analysis showed that the geographical origin of the dominant model samples was positively correlated with the combined OR. Subgroup analysis showed that miR-146a G> C was closely related to the genetic susceptibility to primary hepatocellular carcinoma in the Middle East and Western populations and when PCR-RFLP was used for typing. Non-PCR-RFLP -PCR-RFLP) typing no significant correlation. Sensitivity analysis results for the three genotypes were stable with no published bias. CONCLUSIONS: miR-146a G> C is closely related to the genetic predisposition of primary hepatocellular carcinoma. The individuals with G allele have a higher risk of developing the disease. The genetic polymorphism of this locus has the potential to become a biomarker of primary hepatocellular carcinoma Potential.