论文部分内容阅读
目的观察新生儿脐血单个核细胞(MNC)TLR4、TLR2 mRNA 的表达。方法将46例无窒息新生儿及40例窒息新生儿根据胎龄分组,分离脐血 MNC,测定其 TLR4/2 mRNA 表达及上清中 TNF-α水平。另外将 TLR4/2 mRNA 表达水平与 TNF-α水平进行相关分析。结果无窒息新生儿中足月儿TLR4/2 mRNA 及 TNF-α水平分别为0.75±0.12、0.63±0.08、2502.6±273.1 ng/L,胎龄≥32周但<37周早产儿分别为0.37±0.04、0.32±0.03、1218.8±145.7 ng/L,胎龄<32周早产儿分别为0.26±0.03、0.20±O.03、811.8±105.2 ng/L;窒息新生儿中足月儿 TLR4/2 mRNA 及 TNF-α水平分别为0.58±0.07、0.50±0.06、1946.4±244.2 ng/L,胎龄≥32周但<37周早产儿分别为0.29±0.03、0.26 0.03、970.0±94.3 ng/L,胎龄<32周早产儿分别为0.17±0.02、0.14±0.02、652.6±60.3 ng/L;成人 TLR4/2 mRNA 及 TNF-α水平分别为2.71±0.75、2.61±0.33、9270.1±1098.3 ng/L。早产儿、足月儿 TLR4/2 mRNA 及 TNF-α水平均低于成人,胎龄越低,TLR4/2 mRNA 及 TNF-α水平越低。窒息新生儿 TLR4/2 mRNA 及 TNF-α的表达水平均低于同胎龄无窒息新生儿(P<0.01)。TLR4/2 mRNA 表达水平与 TNF-α水平呈正相关关系。结论新生儿,特别是早产儿,TLR 水平低下,可能是新生儿天然免疫能力低下,容易患败血症等严重感染性疾病的重要原因之一。
Objective To observe the expression of TLR4 and TLR2 mRNA in neonatal cord blood mononuclear cells (MNC). Methods 46 MNCs without asphyxia and 40 asphyxiated neonates were divided into two groups according to their gestational age. The expression of TLR4 / 2 mRNA and TNF-α in the supernatant were determined. In addition, TLR4 / 2 mRNA expression levels were correlated with TNF-α levels. Results The level of TLR4 / 2 mRNA and TNF-α in full-term infants without asphyxia were 0.75 ± 0.12,0.63 ± 0.08,2502.6 ± 273.1 ng / L, gestational age≥32weeks, but <37 weeks preterm infants were 0.37 ± 0.04,0.32 ± 0.03,1218.8 ± 145.7 ng / L, gestational age <32 weeks preterm infants were 0.26 ± 0.03,0.20 ± O.03, 811.8 ± 105.2 ng / L, respectively; full-term neonates with asphyxia TLR4 / 2 mRNA And TNF-α levels were 0.58 ± 0.07,0.50 ± 0.06 and 1946.4 ± 244.2 ng / L, gestational age≥32 weeks but <37 weeks preterm infants were 0.29 ± 0.03,0.26 0.03,970.0 ± 94.3 ng / L and fetal The preterm infants <32 weeks were 0.17 ± 0.02,0.14 ± 0.02,652.6 ± 60.3 ng / L, the levels of TLR4 / 2 mRNA and TNF-α in adults were 2.71 ± 0.75,2.61 ± 0.33,9270.1 ± 1098.3 ng / L, respectively. The levels of TLR4 / 2 mRNA and TNF-α in term infants were lower than those in adults, and the lower the gestational age, the lower the TLR4 / 2 mRNA and TNF-α levels were. The expression levels of TLR4 / 2 mRNA and TNF-α in neonates with asphyxia were lower than those in neonates without asphyxia (P <0.01). TLR4 / 2 mRNA expression levels and TNF-α levels were positively correlated. Conclusion Neonates, especially premature babies, have low TLR levels and may be one of the important causes of severe infectious diseases such as low natural immunity and susceptibility to sepsis in newborns.