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利用SSR标记分析陆地棉野生种系的遗传多样性,对材料间相似系数的变异系数进行显著性测验和矩阵相关性测验,探讨引物和多态性位点数对研究结果准确性的影响。90对多态性引物在42份供试材料间共检测出530个等位位点,其中多态性位点440个,占83.01%。多态信息含量范围为0.046～0.888,平均为0.649;Shannon多样性指数在0.113～2.289之间变动,平均为1.248。显著性测验显示,当引物按PIC值降序排列时,利用25对引物或者150个多态性位点即可获得较准确的结果;升序排列时,至少需要50对引物或200个多态性位点才能获得较准确的结果。矩阵相关性测验显示,降序时20对、升序时50对引物或者达到150个多态性位点聚类即可达到90对引物时的精度。此外,在引物量较少时,扩增位点数较多的引物所提供的信息量更大,随着引物量的增加,这种差距趋于不明显;等位位点总数较少时,引物数量更重要,随着位点数的增加,引物信息含量的重要性已高于引物数起主导作用。综上,若要客观反映出42份陆地棉野生种系的遗传关系,有必要选用多态性引物30对,扩增多态性位点150个以上,增加引物到50对以上为佳。
SSR markers were used to analyze the genetic diversity of upland cotton germplasm. The coefficient of variation (CV) of similarity coefficient between materials was tested by significance test and matrix correlation test, and the influence of primer and polymorphic loci on the accuracy of the study was discussed. A total of 530 alleles were detected among the 90 pairs of polymorphic primers among the 42 tested materials, of which 440 were polymorphic (83.01%). The polymorphic information content ranged from 0.046 to 0.888 with an average of 0.649. Shannon’s diversity index varied from 0.113 to 2.289 with an average of 1.248. The significance test shows that when the primers are arranged in decreasing order of PIC value, more accurate results can be obtained by using 25 pairs of primers or 150 polymorphic loci; in ascending order, at least 50 pairs of primers or 200 polymorphic positions Point to get more accurate results. The matrix correlation test showed that the accuracy of 90 pairs of primers can be achieved when 20 pairs are in descending order, 50 pairs of primers in ascending order or 150 polymorphic loci are clustered. In addition, primers with more amplification sites provided more information when the amount of primer was smaller, and the difference became less obvious with the increase of primer amount. When the total number of alleles was small, primers The quantity is more important. As the number of loci increases, the importance of the information content of the primer has been higher than that of the primer. In summary, if it is necessary to objectively reflect the genetic relationship of 42 wild G. hirsutum germplasms, it is necessary to select 30 pairs of polymorphic primers and to amplify more than 150 polymorphic loci. It is better to increase the number of primers to 50 pairs or more.